Solutions to Your Challenges in Growing and Repairing Tissue



Building with biology using engineered living materials, focuses on programming DNA to grow materials to specifications at the sites where they are needed and developing living materials that are responsive to their environments and can heal when damaged.


Your current research faces challenges that once injected into damaged tissues, stem cells are exposed to acute ischemia and oxygen deprivation, which lead to the production of highly oxidizing compounds, known as reactive oxygen species (ROS). Excessive ROS results in the apoptosis of the transplanted cells. Similarly, exposure of stem cells to extreme culture conditions in vitro (such as starvation, metabolic alterations, and exposure to toxic molecules) also leads to the apoptosis mediated by ROS . Thus, ROS has been recognized as pathological metabolic agents that reduce stem cell functions.  Methods for the in situ detection of ROS levels and the specific species that are required to accurately quantify and characterize the threshold level of ROS to modulate stem cell homeostasis are critical to creating predictable, reproducible therapies.  Combined with accurate ROS measurement of the biochemical and biomechanical environments, stem cells can be modulated by redox status for maximum theraputic outcomes,  XCIR technology leads to reproducible redox therapies to achieve controlled proliferation and differentiation of stem cells towards unlimited biomedical applications


Cell sheet engineering has been developed as an alternative approach to improve mesenchymal stem cell-mediated tissue regeneration. XCIR was capable of modulating epigentics, inducing telomerase activity in stem cells, leading to the up-regulated expression of extracellular matrix type I collagen, fibronectin, and integrin β1, stem cell markers Oct4, Sox2, and Nanog as well as osteogenic markers RUNX2, ALP, OCN.  Under XCIR treatment, stem cells can form cell sheet structures because of increased ECM matrix production.   XCIR modulated mesenchymal stem cells sheets demonstrated a significant improvement in tissue regeneration compared with untreated control dissociated mesenchymal stem cells and offered an effective treatment for tissue defects.   In addition, bone marrow mesenchymal stem cell sheets and umbilical cord mesenchymal stem cell sheets were also well constructed using this method. The development of XCIR mediated mesenchymal stem cell sheets may provide an easy and practical approach for cell-based tissue regeneration.  Contact us for samples of XCIR to acheive the breakthrough outcomes you are working towards.


Ocis has made unprecedented progress in modulating epigenetics of stem cells to solve the challenges that have long obstructed the journey to your research in autologous stem cell technologies .  XCIR is proven to improve the poor survival and longevity of engrafted MSCs.   Ocis has develop novel therapeutic strategies with XCIR technology stem cell modulators for intracellular signal triggering and niche activation.    XCIR is the promising therapeutic strategy of priming therapeutic MSCs  before transplantation.  

© 2020 Ocis Biotechnology